5–5 μm wide, 8–17 μm long Free akinetes with thin coarse cell wa

5–5 μm wide, 8–17 μm long. Free akinetes with thin coarse cell wall, mostly cylindrical, sometimes bent, pale olive, tan, or yellow-orange, with coarsely granulated content, 5–10 μm wide, 12–28 μm long. Reference strain CCALA 1001. Herbarium voucher BRY37722, sequence KF052617. Isolated from Big Horn Seep, Grand Staircase-Escalante National Monument, Utah, USA. This isolate matches selleckchem the description of C. marchicum (Geitler 1932, p. 823) well.

The only difference is the reported colorless content of akinetes, which was not observed in strains from Grand Staircase-Escalante National Monument. The habitat also matches as well, as it was originally found in aerial habitats in Northern Germany and Latvia, and Big

Horn Seep is a relatively dry hanging garden. Cylindrospermum www.selleckchem.com/products/idasanutlin-rg-7388.html moravicum Johansen et Lukešová sp. nov. (Fig. 5, a–o), Thallus gelatinous to leathery, blue-green in young cultures, becoming slightly yellowish with age, with wet-like surface. Trichomes short or long, flexuous, constricted at the cross walls, isopolar, or heteropolar, slightly motile, 2.7–5 μm wide. Vegetative cells cylindrical, sometimes concave or irregular, isodiametric to longer than wide, pale blue-green, 3.5–7.0 μm long. End cells rounded. Heterocytes forming terminally after trichome fragmentation, solitary, unipored, spherical to cylindrical elongated, with yellow, smooth content, 5.0–9.0(11) μm long, 2.7–6.0 μm wide. Akinetes forming paraheterocytically, solitary, cylindrical, widened toward the end attached to the heterocyte, with colorless to golden-brown, smooth, internally structured or lamellate exospores, (18)22–32 μm long, 9–13 μm wide. Holotype: BRY37714, Monte L. Bean Museum, Provo, Utah. Reference strain: CCALA 993. Sequences available

at NCBI GenBank under numbers KF052607 and KF052608 (operons 1 and 2 respectively). Type locality: cave sediment, Amatérská Cave, Moravian Karst, Czech Republic. Etymology: moravicum = from Moravia. Taxonomic Notes: Differs from all other species in this study by the finely structured exospore, which has a hairy appearance but lacks external manifestation of the hairs/spines, and the apically widened Nintedanib (BIBF 1120) cylindrical akinetes. Also differs from these taxa in the secondary structure of the D1-D1′ helix and V3 helix. Cylindrospermum muscicola SAG 44.79 (Fig. 6, t–w) Colony dark green, with small clusters of biomass, dull surface. Filaments long, coiled, unsheathed. Trichomes motile, constricted at cross walls, 4–5 μm wide. Vegetative cells cylindrical, dark green or blue-green, with parietal thylakoids and finely granulated content, 3.5–7 μm long. End cells rounded. Heterocytes terminal, spherical, or spherical-elongated, with tan, clear cytoplasm, 4–6 μm wide, 4.5–8 μm long. Akinetes not observed.

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