001) Functionally, the suppression of Rap1b expression was suffi

001). Functionally, the suppression of Rap1b expression was sufficient to decrease cell motility by inhibiting expression of p38 MAPK rather than VEGF or p42/44 ERK in vitro and in vivo. Moreover, there was a significantly positive correlation between Rap1b and

p38 MAPK expression in ESCC tissues. Conclusion: Our results suggest that the Rap1b/p38 MAPK pathway is associated with survival, tumor progression, and metastasis of ESCC patients. Key Word(s): 1. Rap1b; 2. esophageal squamous cell carcinoma; 3. invasion; 4. P38 MAPK Presenting Author: MINGXIN ZHANG Additional Authors: MINGXIN ZHANG, PENGJIANG ZHANG, QI YANG, QINSHENG WEN, JINGJIE WANG Corresponding Author: MINGXIN ZHANG Affiliations: Tangdu Hospital Fourth Military Medical University, Tangdu Hospital Fourth Military Medical University, Dabrafenib ic50 Tangdu Hospital Fourth Military Medical University, Tangdu Hospital Fourth Military Medical University, Tangdu Hospital Fourth Military Medical University Objective: Cancer related inflammation (CRI) is abnormal signal pathway in cancer cell induced by inflammation and plays important role in esophageal squamous cell carcinoma (ESCC). Our previous study found that miR-302b down-regulated see more in ESCC, but the exact role of miR-302b in the regulation of CRI and its molecular mechanism in ESCC is still unclear. Methods: First,

we examined the expression of miR-302b by quantitative RT-PCR in ESCC patient specimens compared to paired

esophagitis tissues and normal esophageal tissues (NET). Then, to determine the possible correlation between miR-302b and CRI signal pathway, ESCC cell lines (EC9706, Eca109, TE1, TE10, TE11, and OE33) were treated with by various inflammation stimulation factors (LPS, IL-6, IFN-γ, and TGF-β). Expression of miR-302b was detected by quantitative selleck screening library RT-PCR and gene profiles were tested by gene microarray. Finally, immunohistochemical staining and western blotting analysis of ESCC specimens were carried out to reveal correlation between miR-302b and miR-302b potential targeted CRI related gene (ERBB4, TGFBR2, CXCR4, and IRF2) expression. Results: Expression of miR-302b showed a trend to decrease form NET to ESCC tissues. After inflammation stimulation, miR-302b decreased. Gene profiles revealed an inflammatory gene signature with upregulation of numerous cancer-related inflammation genes including some miR-302b potential targeted CRI related genes (ERBB4, TGFBR2, CXCR4, and IRF2). Moreover, there was a significantly negative correlation between miR-302b and CRI related genes (ERBB4, TGFBR2, CXCR4, and IRF2) expression in ESCC tissues. Conclusion: Our results suggest that miR-302b plays important role in the CRI of ESCC possibly by regulation expression of CRI related genes (ERBB4, TGFBR2, CXCR4, and IRF2). Key Word(s): 1. miR-302b; 2. esophageal squamous cell carcinoma; 3.

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