Hierarchical clustering of a correlation matrix revealed functional clusters of genes associated with Th17 (RORC, IL17A), Th2 (IL4, IL5, IL13), Th1 (Tbet, IRF1, IL12Rb2, STAT4), and cytotoxicity (GNLY, GZMB, PERF1). High-IL17A mRNA expression level was most frequent at early stages of tumor progression. Patients with high expression of the Th17 cluster had a poor prognosis whereas patients with high expression of the Th1 cluster had prolonged disease-free survival. In contrast no prediction of the prognosis was associated EX 527 in vivo with the Th2 clusters. The combined analysis
of cytotoxic/Th1 and Th17 clusters gave a better discrimination for relapse. In situ analysis of IL17+ cells and CD8+ cells using tissue-microarray confirmed with these results. Conclusion: Functional clusters associated with Th1 and Th17 cells have opposite effect on patients survival and bring complementary information. Poster No. 177 The Effect of hCaMKIINa on TLR4-Triggered Cytokine Production of Colon Cancer Cells
Chunmei Wang 1 , Nan Li1, Xingguang Liu1, Qinghua Zhang1, Xuetao Cao1 1 National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University, Shanghai, China PLX3397 Increasing evidences CFTRinh-172 mouse suggest that chronic inflammation contributes to cancer development and progression. One of the underlying mechanisms is proposed that tumor cell-derived inflammatory and immunosuppressive cytokines contribute to tumor immune escape and resistance to immunotherapy. Isotretinoin Toll-like receptors (TLRs) have been implicated in tumor progression and metastasis. Our previous study showed that calcium/calmodulin-dependent protein kinase II (CaMKII) promoted TLR-triggered proinflammatory cytokine in macrophages. hCaMKIINa, a novel CaMKII inhibitory protein identified by us, suppressed the growth of colon cancer cell by inducing cell cycle arrest in vitro and in vivo. Thus we wonder whether hCaMKIINa-mediated CaMKII inhibition affects TLR4-triggered cytokine production of colon cancer cells for immune escape. In this study, we demonstrate that TLR4 is expressed
on human colon cancer cell lines. TLR4 ligation promotes production of immunosuppressive cytokines IL-8 and VEGF. Overexpression of hCaMKIINa inhibits TLR4-triggered production of IL-8 and VEGF; H282R, constitutive activated CaMKII, significantly promotes TLR4-triggered IL-8 and VEGF secretion. In addition, we also observe that hCaMKIINa inhibits LPS-mediated activation of p-ERK1/2 and LPS-mediated TLR4 expression in SW620 cells. Furthermore, hCaMKIINa-mediated inhibition of ERK1/2 is necessary for suppression of TLR4-triggered IL-8 and VEGF secretion. These results suggest that hCaMKIINa-mediated CaMKII inhibition might play important roles in the suppression TLR4-triggered metastasis and immune escape of human colon cancer cells by inhibiting immunosuppressive cytokine production. Poster No.