These methods can Tyrosine Kinase Inhibitor Library be utilised over time to monitor

trends and can also be applied to birth cohorts and at subnational level, with adequate confidence levels, to explore for heterogeneity of risk [35]. Sero-surveys may also provide useful data to provide estimates of Re and signal the risk of impending outbreaks [37]. It is often disconcerting for public health programmes when the majority of measles cases occur in children too young to have received one or two doses of measles containing vaccine. It is important to note that this generally represents a relative increase in cases in this age-group and not an absolute increase. The immediate temptation is to shift the lower recommended age for vaccination to young infants. Although it may be necessary in specific situations, for example large outbreaks, to provide a supplementary dose of vaccine at 6 months of age this should not replace the dose provided from 8 to 12 months of age, as seroconversion and protection is significantly lower during younger infancy due to maternal antibody interference with the child’s immune response to the vaccine [38]. Similarly measles incidence may increase in older age groups in absolute or relative terms, typically amongst adults

or teenagers who may have been part of the first birth cohorts to inhibitors receive measles containing vaccine. Generally programme coverage builds over time SP600125 mouse and many programmes initiated measles vaccination with only a single dose. Thus it is not surprising that there is often an increased proportion of susceptibles in these age cohorts

and a relatively higher burden of infection amongst these individuals during community outbreaks in areas approaching or having achieved measles elimination. A further conundrum is worth brief mention. IgM serology remains as the backbone of measles laboratory confirmation in most countries. Although these tests, performed in WHO approved laboratories, are generally excellent for programme purposes, like any test they are not 100% specific. In low prevalence elimination environments IgM serology will have a low positive predictive value, i.e. a considerable proportion of tests will provide false positive results. Indeed, if whatever no measles cases are occurring, then all positive test results are expected to be false positives. Other diagnostic tests particularly immunofluorescence, which may be used in the early phases of disease, is particularly prone to high false positivity. Guidelines have been developed to assist in the interpretation of results in these settings but it is particularly important not to view laboratory results in isolation from the clinical presentation, travel history and careful description of contact with possible cases [39].