While NKG2D+CD4+ T cells are
inversely Selleck BVD-523 correlated with disease in juvenile-onset SLE, immunosuppressive NKG2D+CD4+ T cells appear functionally uncompromised, although classic regulatory T cell functions are typically impaired in SLE, this may be clinically significant (29). Because of the positive correlation of NKG2D+CD3+CD8− cells with viral loads, our results suggest that the increased frequency of NKG2D+CD3+CD8− cells observed in HIV infection may impede T cell immune activation during disease progression, possibly resulting in distortions of T cell cytolytic function. Although CD4+ T cells are targeted by HIV, not all CD4+ T cells are infected equally. Resting memory CD4+ T cells are more susceptible to HIV infection than naïve cells (30). It has also been found that CCR5-using (R5) HIV is most efficiently transmitted to central memory T cells and that CXCR4-using (X4) HIV is preferentially transmitted to naïve T cells (31). Moreno-Fernandez
et al. found that circulating regulatory T cells were not preferentially infected with HIV compared to effector T cells in vivo (32). As NKG2D+CD4+ T cells, that produce interleukin-10 and transforming growth factor-β, as well as Fas ligand, which inhibits bystander T cell proliferation in vitro, represent a type of regulatory cells, similar to regulatory T cells. (29). They may be less ABT-888 mw susceptible to HIV infection, resulting in their accumulation during infection. In summary, during PFKL HIV infection we observed an upregulation of NKG2A+NKG2D− T cells among the CD8+ and CD3+CD8− subpopulations,
a downregulation of NKG2D+NKG2A−CD8+T cells, and an upregulation of NKG2D+NKG2A−CD3+CD8− cells. Furthermore, we found that combinational analysis of the expression of inhibitory and activating NKRs on T cells may provide clearer results than analysis of individual NKRs. The mechanisms linking viral replication to dysregulated NKR expression remain obscure, with the function of CD4+NKG2D+ T cells particularly requiring further study. Overall, we conclude that NKR expression on T cells changes with HIV disease progression in a pattern that predicts exacerbated impairment of the immune response to HIV infection. The authors wish to express their gratitude to the patients who participated in this study. This work was supported by a research grant from the Mega Projects of National Science Research for the 12th Five-year Plan (2012ZX10001-006) , 973 Programs about the Development of National Significant Elementary Research (2006CB504206), and the Programme of the Innovative Group of Institutions of Higher Education of the Education Department of Liaoning Province (2008T202). “
“During their development, B lymphocytes undergo V(D)J recombination events and selection processes that, if successfully completed, produce mature B cells expressing a non-self-reactive B-cell receptor (BCR).