Open bars indicate microarray learn more fold-change, solid bars indicate qRT-PCR fold-change. B. melitensis 16 M express different sets of genes in late-log and stationary phases of growth in F12K tissue culture medium Of the 454 genes significantly altered in B. melitensis during late-log phase (14% of B. melitensis genome), 414

(91%) were up- and 40 (9%) were down-regulated, compared to when the bacteria were allowed to reach stationary phase [see Additional file 2]. The relative changes in gene expression ranged from a 386.5-fold induction of the Glycerol-3-phosphate regulon repressor gene (BMEII1093) to a 60.5-fold down-regulation of the locus BMEII0615 (hypothetical protein). As expected, the majority of gene expression changes were associated with growth and metabolism. Among the up-regulated genes were those associated with DNA replication, transcription and translation (57 genes), nucleotide, amino acid, lipid and carbohydrate metabolism (65 genes), energy production and S63845 conversion (24 genes), membrane transport (56 genes) and cell envelope, biogenesis and outer membrane (26

genes), while Meloxicam the 40 down-regulated genes were distributed among several COGs (Figure 4). Figure 4 Distribution of genes differentially expressed at late-log growth phase compared to stationary phase associated in cluster of ortholog genes (COGs) functional categories. Functional classifications are as GSK2118436 follows: A, DNA replication, recombination and repair; B, Transcription; C, Translation, ribosomal structure and biogenesis; D, Nucleotide metabolism; E, Carbohydrate metabolism; F, Lipid metabolism;

G, Amino acid metabolism; H, Secondary metabolites biosynthesis, transport and metabolism; I, Energy production and conversion; J, Inorganic ion transport and metabolism; K, Cofactor transport and metabolism; L, Cell envelope, biogenesis and outer membrane; M, Membrane transport; N, Defense mechanism; O, Signal transduction; P, Post-translational modification and secretion, protein turnover and chaperones; Q, Cell division; R, Cell motility and chemotaxis; S, General function prediction only; T, Predicted by homology; U, Unknown function. Solid bars, up-regulated genes; open bars, down-regulated genes.