PubMedCrossRef 8. Nugent R, Krohn M, Hillier S: Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation. J Clin Microbiol 1991, 29:297–301.PubMed 9. Hugenholtz P, Goebel BM, Pace NR: Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. J Bacteriol 1998, 180:4765–4774.PubMed 10. Sha BE, Chen HY, Wang QJ, Zariffard MR, Cohen MH, Spear GT: Utility of Amsel criteria, Nugent

score, and quantitative PCR for Gardnerella vaginalis, Mycoplasma hominis, and Lactobacillus spp. for diagnosis of bacterial vaginosis in human immunodeficiency virus-infected women. J Clin Microbiol 2005, 43:4607–4612.PubMedCrossRef 11. Verhelst R, Verstraelen H, Claeys G, Verschraegen G, Delanghe J, Van Simaey L, De Ganck C, Temmerman M, Vaneechoutte M: Cloning of 16 S rRNA genes amplified buy SIS3 from normal and disturbed vaginal microflora suggests a strong association between Atopobium vaginae, Gardnerella vaginalis and bacterial vaginosis. BMC Microbiol 2004, 4:16.PubMedCrossRef 12. Fredricks DN, Fiedler TL, Thomas KK, Oakley BB, Marrazzo JM: Targeted PCR for detection of vaginal bacteria associated with

bacterial vaginosis. J Clin Microbiol 2007, 45:3270–3276.PubMedCrossRef 13. Hummelen R, Fernandes Navitoclax AD, Macklaim JM, Dickson RJ, Changalucha J, Gloor GB, Reid G: Deep sequencing of the vaginal microbiota of women with HIV. PLoS One 2010, 5:4-Hydroxytamoxifen purchase e12078.PubMedCrossRef 14. Ravel J, Gajer P, Abdo Z, Schneider GM, Koenig SS, McCulle SL,

Karlebach S, Gorle R, Russell J, Tacket CO, Brotman RM, Davis CC, Ault K, Peralta L, Forney LJ: Vaginal microbiome of reproductive-age women. Proc Natl Acad Sci USA 2011,108(Suppl 1):4680–4687.PubMedCrossRef 15. Spear GT, Gilbert D, Landay AL, Zariffard R, French AL, Patel P, Gillevet PM: Pyrosequencing of the genital microbiotas of HIV-seropositive and Thiamine-diphosphate kinase -seronegative women reveals Lactobacillus iners as the predominant Lactobacillus Species. Appl Environ Microbiol 2011, 77:378–381.PubMedCrossRef 16. Zhou X, Brown CJ, Abdo Z, Davis CC, Hansmann MA, Joyce P, Foster JA, Forney LJ: Differences in the composition of vaginal microbial communities found in healthy Caucasian and black women. ISME J 2007, 1:121–133.PubMedCrossRef 17. Lamont R, Sobel J, Akins R, Hassan S, Chaiworapongsa T, Kusanovic J, Romero R: The vaginal microbiome: new information about genital tract flora using molecular based techniques. BJOG 2011, 118:533–549.PubMedCrossRef 18. Srinivasan S, Liu C, Mitchell CM, Fiedler TL, Thomas KK, Agnew KJ, Marrazzo JM, Fredricks DN: Temporal variability of human vaginal bacteria and relationship with bacterial vaginosis. PLoS One 2010, 5:e10197.PubMedCrossRef 19. Verstraelen H, Verhelst R, Claeys G, De Backer E, Temmerman M, Vaneechoutte M: Longitudinal analysis of the vaginal microflora in pregnancy suggests that L. crispatus promotes the stability of the normal vaginal microflora and that L. gasseri and/or L.