Species rich in phenolic compounds, ascorbic acid and carotenes are usually associated with prominent biological properties such as increased protection against cellular oxidation, antimicrobial

and anticarcinogenic activities ( Katalinić et al., 2010, Link et al., 2010, Proteggente et al., 2002 and Sun et al., 2002). The beneficial effects of fruit and vegetable consumption in the prevention of chronic-degenerating diseases have been challenged ( Boffetta et al., 2010). However, the majority of studies suggest that increased consumption of fruit, vegetables and grains contributes to prevent chronic-degenerating diseases, such as cancer, cardiovascular diseases, diabetes and neurodegenerative diseases ( Bazzano et al., 2002, Liu et al., 2004 and Schroeter et al., 2005). In this study, fruit from six araçá genotypes (accessions) were characterised MAPK inhibitor by quantification of individual phenolic

compounds, l-ascorbic acid, total phenolic, total anthocyanin, and total carotene content. Acetone and aqueous fruit extracts were analysed in terms of radical scavenging power, antioxidant protection of Saccharomyces cerevisiae, antimicrobial effect against Salmonella enteritidis and antiproliferative effect on human cancer cells, MCF-7 (breast) and Caco-2 (colon). Red (accessions buy Staurosporine AR9, AR19 and AR29) and yellow (accessions AR27, AR46 and AR72) araçá (P. cattleianum Sabine) were collected from a research orchard (germplasm collection of Embrapa Clima Temperado, Pelotas, RS, Brazil) when fruit was ripe. One kilogram of fruit selleck from three plants (clones) of each accession was harvested. Fruit were washed, seeds removed, and fruit flesh was frozen

in liquid nitrogen and stored at −80 °C until further analyses. All analyses were performed in triplicate. Soluble solids content was determined by refractometry, and the results expressed as % (w/w). Total acidity (TA) and pH were measured directly from the extracted fruit juice. TA was determined by titration and results were expressed as milligrams of citric acid per 100 grams of fresh fruit pulp (mg 100 g-1 ffp). Phenolic compounds were extracted following the method described by Souza et al. (2008). Frozen pulp (10 g) was ground in a mortar and pestle, extracted with 20 mL deionized water (DW) and placed on an orbital shaker set at 200 rpm for 1 h at room temperature (20 ± 3 °C) in the dark. Extracts were then centrifuged at 12000g for 15 min at 4 °C and the supernatant was concentrated in a freeze-drier and the final volume adjusted to 10 mL with DW. The same extraction was performed using acetone instead of water. In this case the extract was concentrated in a rotary evaporator at 40 °C under reduced pressure and the residue was redissolved in 10 mL of DW. Total phenolic content was determined using the method described by Dewanto, Wu, Adom, and Liu (2002).