bTest results of both the API-20E system and conventional test NVP-LDE225 methods. cThe carbon source utilization tests were determined by using Biolog GN2 microplates. *Species: 1, Enterobacter oryziphilus sp. nov. (n=3); 2, Enterobacter oryzendophyticus sp. nov. (n=3); 3, Enterobacter radicincitans D5/23T; 4, Enterobacter turicensis 508/05T; 5, Enterobacter helveticus 513/05T; 6, Enterobacter pulveris 601/05T. 7, Enterobacter cloacae subsp. cloacae; data from [23–25]; 8, Enterobacter cloacae subsp. dissolvens, data from

[8, 26]. The percentage of strains giving a positive result is scored as: -, 0–20%; V, 20–80%; +, 80–100%; ND, no data available; cell morphology: R, rods; CR, coccoid rods; SR, straight rods. The putative type strains REICA_142T and REICA_082T were then compared to the API20E database. The database revealed as closest relative for the group-I type

strain REICA_142T Enterobacter asburiae (only 29% identity) and for group-II type strain REICA_082T E. cloacae (95% identity), respectively. The two strains differed mainly in the ornithine decarboxylase test and in the production of acid from L-rhamnose and selleck chemicals llc D-melibiose (all reactions positive in strain JNK-IN-8 price REICA_082T). However, this database is as limited as the MIDI one discussed earlier,

Demeclocycline and environmental strains are needed to make it suitable for environmental work. Plant-beneficial and adaptive traits Finally, we evaluated the capacities of strains REICA_142T (group-I) and REICA_082T (group-II) to modulate rice plant growth and to colonize rice host plants from soil. Group-II strain REICA_082T produced indole acetic acid (IAA; 4.12 μg ml-1; ±0.68) from L-tryptophan, whereas group-I strain REICA_142T did not. Both strains revealed the production of acetoin, 2-ketogluconate via gluconate dehydrogenase and siderophores (after 24 h at 30°C), and the solubilisation of phosphate via acidification but not alkalinisation. 2-ketogluconate is the salt compound of the organic acid 2-ketogluconic acid. This organic acid is produced by phosphate-solubilizing bacteria (PSB) and is known to be involved in the solubilisation of inorganic phosphates [27].