Table 2 Validation Fludarabine of microarray data using qRT-PCR of randomly selected genes relative to the housekeeping gene, rpoD a Locusb Namec Primer sequenced Fragment (bp)e Serovar Typhimurium Gene see more Functionf Ratio of arcA mutant/WT Log2ratio           qRT-PCR g Microarray h qRT-PCR i Microarray j STM3217 aer 5′-CGTACAACATCTTAATCGTAGC-3′ 5′-TTCGTTCAGATCATTATTACCC-3′ 163 aerotaxis sensor receptor, senses cellular redox state or proton motive force 0.237 0.293 -2.1 -1.8 STM1919 cheM 5′-GCCAATTTCAAAAATATGACG-3′

5′-GTCCAGAAACTGAATAAGTTCG-3′ 114 methyl accepting chemotaxis protein II, aspartate sensor-receptor 0.194 0.261 -2.4 -1.9 STM0441 cyoC 5′-TATTTAGCTCCATTACCTACGG-3′ 5′-GGAATTCATAGAGTTCCATCC-3′ 134 cytochrome o ubiquinol oxidase subunit III 4.920 5.465 2.3 2.5 STM1803 dadA 5′-TAACCTTTCGCTTTAATACTCC-3′ 5′-GATATCAACAATGCCTTTAAGC-3′ 155 D-amino acid dehydrogenase subunit 3.430 10.520 1.8 3.4 STM2892 invJ 5′-TTGCTATCGTCTAAAAATAGGC-3′ 5′-TTGATATTATCGTCAGAGATTCC-3′ 128 surface presentation of antigens; secretory proteins 0.855 1.010 -0.2 0.0 STM2324 nuoF 5′-GGATATCGAGACACTTGAGC-3′ 5′-GATTAAATGGGTATTACTGAACG-3′ 163 NADH dehydrogenase I chain F 0.380 1.706 -1.4 0.8 STM0650 STM0650 5′-CAACAGCTTATTGATTTAGTGG-3′ 5′-CTAACGATTTTTCTTCAATGG-3′ 130 putative hydrolase C-terminus 0.274 0.123 -1.9 -3.0 STM2787 LY3039478 STM2787 5′-AAGCGAATACAGCTATGAACC-3′

5′-ATTAGCTTTTGCAGAACATGG-3′ 144 tricarboxylic transport 6.440 90.770 2.7 6.5 STM4463 STM4463 5′-AAGGTATCAGCCAGTCTACG-3′ 5′-CGTATGGATAAGGATAAATTCG-3′ 142 putative arginine repressor 0.165 0.012 -2.6 -6.4 STM2464 eutN 5′-AGGACAAATCGTATGTACCG-3′ 5′-ACCAGCAGTACCCACTCTCC-3′ 153 putative detox protein in ethanolamine utilization 0.181 0.159 -2.5 -2.7 STM2454 eutR 5′-GGTAAAAGAGCAGCATAAAGC-3′ 5′-ATTATCACTCAAGACCTTACGC-3′ 118 putative regulator ethanolamine operon (AraC/XylS Dehydratase family) 0.189 0.188 -2.4 -2.4 STM2470 eutS 5′-AATAAAGAACGCATTATTCAGG-3′

5′-GTTAAAGTCATAATGCCAATCG-3′ 137 putative carboxysome structural protein, ethanol utilization 0.197 0.105 -2.3 -3.3 STM1172 flgM 5′-AGCGACATTAATATGGAACG-3′ 5′-TTTACTCTGTAAGTAGCTCTGC-3′ 126 anti-FliA (anti-sigma) factor; also known as RflB protein 0.196 0.163 -2.4 -2.6 STM3692 lldP 5′-TGATTAAACTCAAGCTGAAAGG-3′ 5′-CCGAAATTTTATAGACAAAGACC-3′ 189 LctP transporter, L-lactate permease 5.950 12.780 2.6 3.7 STM3693 lldR 5′-GAACAGAATATCGTGCAACC-3′ 5′-GAGTCTGATTTTCTCTTTGTCG-3′ 153 putative transcriptional regulator for lct operon (GntR family) 5.750 80.000 2.5 6.3 STM1923 motA 5′-GGTTATCGGTACAGTTTTCG-3′ 5′-TAGATTTTGTGTATTTCGAACG-3′ 194 proton conductor component of motor, torque generator 0.282 0.253 -1.8 -2.0 STM4277 nrfA 5′-GACTAACTCTCTGTCGAAAACC-3′ 5′-ATTTTATGGTCGGTGTAGAGC-3′ 159 nitrite reductase periplasmic cytochrome c(552) 0.314 0.285 -1.7 -1.8 aSTM3211 (rpoD) is a housekeeping gene that was used as the reference gene where no significant change in expression level was observed.