Whereas anti-vaccine CTL were rare in the blood and inside metastases of this patient, anti-tumor CTL recognizing other tumor Ags, mainly MAGE-C2, were 100 times more frequent in the blood and considerably enriched in metastases following vaccination. In this study we report the analysis of the CTL response of a second melanoma patient who showed a mixed tumor response after vaccination with dendritic cells pulsed with two MAGE-A3 antigenic peptides presented, respectively, by HLA-A1 and HLA-DP4. Anti-MAGE-3.A1 CD8 and anti-MAGE-3.DP4 CD4 T cells became detectable in the blood after
vaccination at a frequency of similar to 10(-5) among the CD8 or CD4 T cells, respectively, and they were slightly enriched PND-1186 order in slowly progressing metastases. Additional anti-tumor CTL were present in the blood at a frequency of 2 x 10(-4) among the CD8 T cells and, among these, Acalabrutinib price an anti-MAGE-C2 CTL clone was detected only following vaccination and was enriched by > 1,000-fold in metastases relative to the blood. The striking similarity of these results with our previous observations further supports
the hypothesis that the induction of a few anti-vaccine T cells may prime or restimulate additional anti-tumor T cell clones that are mainly responsible for the tumor regression.”
“Salivary proteins influence the biomineralization of hydroxyapatite (HAp) within enamel. Their effect on the crystal growth has been extensively studied, but, their effect on demineralization kinetics is less well investigated. In this study bovine serum albumin (BSA) was used as a
model protein to measure its effect on demineralization kinetics of hydroxyapatite aggregates using scanning microradiography (SMR). HAp aggregates (8 and 20% porous) were cut into 5 x 5 x 2 mm blocks. SMR cells were prepared containing hydroxyapatite blocks. BSA was added to demineralising solutions 10.1 mol L(-1) acetic acid, buffered to pH 4.0; degree of saturation zero Belnacasan Apoptosis inhibitor and 0.062 respectively) at a concentration range 0.76-75.8 mu mol L(-1). Demineralising solution without added BSA was used as a control. The demineralising solutions were circulated past the samples at 0.4 mL min(-1). SMR was used to measure the rate of mineral loss (RMLHAp) at 14 points in each sample repeatedly for 3 weeks. The results show that BSA increases or decreases the RMLHAp depending on; BSA concentration, HAp porosity, and the degree of saturation of the demineralising solution. It is suggested that BSA influences demineralization kinetics of HAp either by modifying solution properties, or, by affecting the surface energy of hydroxyapatite. (C) 2010 Wiley Periodicals, Inc.